Current Issue : April - June Volume : 2019 Issue Number : 2 Articles : 6 Articles
A simple and sensitive liquid chromatographyâ??quadrupole-time-of-flightâ??mass\nspectrometric (LC-QTOF-MS) assay has been developed for the evaluation of drug metabolism\nand pharmacokinetics (PK) properties of vipadenant in rat, a selective A2a receptor antagonist\nas one of the novel immune checkpoint inhibitors. A simple protein precipitation method using\nacetonitrile was used for the sample preparation and the pre-treated samples were separated by\na reverse-phase C18 column. The calibration curve was evaluated in the range of.............
Precise and sophisticated HPTLC and HPLC methods were developed for determination of caffeine from coffee bean extract. HPTLC analysis was performed on silica gel 60 F254 plate using ethyl acetate: methanol: water in the proportion of 10: 1.4: 1 (v/v/v) as mobile phase. A clear band of caffeine was observed at Rf value 0.55 (+0.1). Caffeine shows linearity within the concentration range of 200-600 ppm with regression coefficient of 0.9987, at 254 nm. HPLC analysis was performed by using mobile phase of methanol and water (40:60) with Zorbax SB C-18 column. Quantification was carried out by using PDA detector at 272nm. The method was validated in terms of specificity, precision, linearity, robustness, accuracy, limit of detection (LOD) and limit of quantification (LOQ) as per ICH Q2 (R1) guidelines. The % RSD for validation parameters including accuracy, method precision, intermediate precision, robustness was found to be less than 2.0 %. In the present study, accurate and sophisticated HPTLC and HPLC methods were validated for determination of caffeine in coffee bean extract. Also qualitative analysis was performed for the estimation of caffeine from prepared capsule formulation using HPTLC method....
A simple and sensitive liquid chromatographic method was developed for the estimation of darifenacin hydrobromide in liposomes. Analysis was carried out on Enable 18H C18 column using a mobile phase consisting of phosphate buffer pH 3 and acetonitrile (60:40 %v/v). The mobile phase was pumped at a flow rate of 1 ml/min and UV-detection was carried out at 284 nm. Linearity was observed over concentration range of 5-30 µg/ml and the coefficient of determination was found to be 0.9999. The proposed method was validated as per ICH Q2 (R1) guidelines. The limit of detection and limit of quantitation were found to be 0.24 and 0.73 ng/ml. The method was found to be linear, sensitive, precise, accurate and robust. The method has shown consistent and good recoveries from liposomes (98.96±0.88%) and tablet formulation (100.43±0.24%). The developed method was successively used for the quantitative analysis of darifenacin hydrobromide in the prepared liposomes....
Guinep is traditionally used in the management of cardiovascular ailments. This study\naims to evaluate its medicinal constituents and effects in the management of myocardial injury in\nan experimental isoproterenol (ISO) rat model. Sprague-Dawley rats were randomly assigned to four\ngroups: Group 1 was the control group; Group 2 received M. bijugatus extract (100 mg/Kg; MB) for\nsix weeks; Group 3 was given ISO (85 mg/Kg) i.p. twice during a 24-hour period; and Group 4 was\ngiven ISO (85 mg/Kg) i.p. and MB extract (100 mg/Kg) for six weeks. The MB was administered\norally by gavage, daily. The blood pressure of conscious animals was measured, while ECG was\nperformed under anesthesia. Blood and serum were collected for biochemical and hematological\nanalysis. The ISO group treated with MB showed a significant decrease (p < 0.001) in (SBP), diastolic\n(DBP), mean arterial (MAP) and heart rate (HR) compared to the ISO only group. Conversely, MB\ntreated rats that were not induced with ISO displayed a significant decreases (p < 0.001) in SBP, DBP,\nMAP, and HR. ISO significantly elevated the ST segment (p < 0.001) and shortened the QTc interval\n(p < 0.05), which were recovered after treatment with 100 mg/Kg of MB. In addition, the results\nshowed a significant decrease (p < 0.001) in the heart to body weight ratio of the ISO group treated\nwith MB compared to the ISO only group. Furthermore, the extract normalized the hematological\nvalues depressed by the ISO while significantly elevating the platelet count. UHPLC high-resolution\norbitrap mass spectrometry analysis results revealed the presence of several antioxidants like vitamin\nC and related compounds, phenolic acids, flavonoid, fatty acids (oxylipins), and terpene derivatives.\nThe results of this study indicated that Melicoccus bijugatus did display some cardio-protective effects\nin relation to myocardial injury....
Natural deep eutectic solvents (NADESs), composed of natural primary metabolites,\nare now widely used as green and sustainable extraction solvents of bioactive components.\nIn the present study, various NADESs were prepared to extract multi-components from different\npreparations of an herbal formula (Chinese name: Jinqi Jiangtang, JQJT) using ultrasound-assisted\nextraction (UAE). Results showed that most prepared NADESs provided more effective extraction of\nphenolic acids and alkaloids from JQJT preparations than conventional solvents. Among the tested\nNADESs, the solvent formed by choline chloride and laevulinic acid was selected to optimize the\noperational parameters using response surface methodology. The optimized extraction method\nwas successfully applied to extract six major components in four commercial JQJT products,\nand quantification analysis was performed by the validated high-performance liquid chromatography\nwith ultraviolet detection (HPLC-UV) method. The quantitative results indicated that preparations\nfrom different manufacturers showed different chemical profiles. In conclusion, NADESs-based\nUAE shows considerable potential as an efficient and green method for extraction of multi-bioactive\ncomponents from commercial herbal preparations....
This work concerns with the development and validation of a simple, specific and cost effective RP-HPLC method for simultaneous estimation of ribociclib and palbociclib in bulk samples. Chromatography was carried on Kromasil C18 column with mobile phase comprising of 20 mM phosphate buffer (pH-5): methanol: acetonitrile (40:30:30, v/v/v). The flow rate was adjusted to 1 ml/min with PDA detection at 260 nm. The retention times of ribociclib and palbociclib were found to be 3.47min and 4.48 min, respectively and other replicate standard system suitability parameters are within the limit and uniform. The different analytical parameters such as accuracy, precision, linearity, robustness, limit of detection (LOD), limit of quantification (LOQ) were determined according to the International Conference on Harmonization (ICH) Q2B guidelines. The detector response was linear in the range of 5-25 mcg/ml and 1-5 mcg/ml for ribociclib and palbociclib respectively....
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